LOCUS       pLV-[hsa-miR-29c]        9142 bp    DNA     circular     15-MAR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7063..7923
                     /label=Amp(R)
     promoter        8124..8664
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5668..5902
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6477..6932)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5974..6104
                     /label=SV40 pA
     misc_feature    6105..6318
                     /label=SV40 ori
     misc_feature    4989..5585
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3607
                     /label=intron
     CDS             3654..4979
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3397
                     /label=hsa-mir-29c precursor
BASE COUNT     2163 a      2373 c      2494 g      2112 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcca gaggatgccc 
     3121 tggagtattc ggcgttgatt gcggggcacc cgaaatcaga cttgccacct ggactgtcga 
     3181 ggtgcagacc ctgggagcac cactggccca tctcttacac aggctgaccg atttctcctg 
     3241 gtgttcagag tctgtttttg tctagcacca tttgaaatcg gttatgatgt agggggaaaa 
     3301 gcagcagcct cgaagcctca tgccaactct gggcagcagc agcctgtggt ttcctggaag 
     3361 atggatgggc agagaatagg gaaggaagat catgcttgct agctcgagct tttggagtac 
     3421 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt 
     3481 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt 
     3541 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc 
     3601 atttcaggtg tcgtgaaaac tacccctcta gagtcgagct accggtcgcc accatggtga 
     3661 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca 
     3721 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct 
     3781 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct 
     3841 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg 
     3901 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga 
     3961 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt 
     4021 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga 
     4081 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga 
     4141 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca 
     4201 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca 
     4261 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg 
     4321 agggccgcca ctccaccggc ggcatggacg agctgtacaa ggacccaccg gtcgccacca 
     4381 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac 
     4441 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc 
     4501 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 
     4561 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 
     4621 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 
     4681 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc 
     4741 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 
     4801 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 
     4861 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 
     4921 acgtcgagtg cccgaaggac cgcgcgacct ggtgcatgac ccgcaagccc ggtgcctgag 
     4981 cggccgcgtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 
     5041 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 
     5101 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 
     5161 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 
     5221 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 
     5281 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 
     5341 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 
     5401 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 
     5461 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 
     5521 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 
     5581 gcctggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 
     5641 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa 
     5701 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 
     5761 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 
     5821 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 
     5881 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 
     5941 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 
     6001 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 
     6061 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc 
     6121 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 
     6181 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 
     6241 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga 
     6301 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 
     6361 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 
     6421 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc 
     6481 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 
     6541 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 
     6601 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 
     6661 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 
     6721 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 
     6781 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 
     6841 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 
     6901 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc 
     6961 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 
     7021 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 
     7081 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 
     7141 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 
     7201 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 
     7261 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 
     7321 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 
     7381 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 
     7441 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 
     7501 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 
     7561 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 
     7621 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 
     7681 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 
     7741 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 
     7801 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 
     7861 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 
     7921 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 
     7981 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 
     8041 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 
     8101 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 
     8161 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 
     8221 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 
     8281 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 
     8341 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 
     8401 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 
     8461 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 
     8521 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 
     8581 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 
     8641 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 
     8701 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 
     8761 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 
     8821 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 
     8881 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 
     8941 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 
     9001 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 
     9061 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa 
     9121 agggaacaaa agctggagct gc 
//
